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Efficient transformation of the beetle Tribolium castaneum using the Minos transposable element
Abstract
Genetic transformation in insects holds great promise as a tool for genetic manipulation in species of particular scientific, economic, or medical interest. A number of transposable elements have been tested recently as potential vector...
Publication · 3h
Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis
Abstract
The amphipod crustacean Parhyale hawaiensis has been put forward as an attractive organism for evolutionary developmental comparisons, and considerable effort is being invested in isolating developmental genes and studying their express...
Publication · 3h
A versatile strategy for gene trapping and trap conversion in emerging model organisms
Abstract
Genetic model organisms such as Drosophila, C. elegans and the mouse provide formidable tools for studying mechanisms of development, physiology and behaviour. Established models alone, however, allow us to survey only a tiny fraction o...
Publication · 3h
Clonal analysis by tunable CRISPR-mediated excision
Abstract
ABSTRACT Clonal marking techniques based on the Cre/lox and Flp/FRT systems are widely used in multicellular model organisms to mark individual cells and their progeny, in order to study their morphology, growth properties and developme...
Publication · 3h
A common cellular basis for muscle regeneration in arthropods and vertebrates
Abstract
Crustacean limb regeneration relies on committed progenitor cells, including satellite-like muscle precursors. Many animals are able to regenerate amputated or damaged body parts, but it is unclear whether different taxa rely on similar...
Publication · 3h
DNA sequence evolution: the sounds of silence.
Abstract
Silent sites (positions that can undergo synonymous substitutions) in protein-coding genes can illuminate two evolutionary processes. First, despite being silent, they may be subject to natural selection. Among eukaryotes this is exempl...
Publication · 3h
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